Glutamine synthetase activity fuels nucleotide biosynthesis and supports growth of glutamine-restricted glioblastoma
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منابع مشابه
Assay for glutamine synthetase activity.
The use of phosphoenolpyruvate plus pyruvate kinase as an ATP-generating system in the assay for glutamine synthetase activity via the formation of gamma-glutamylhydroxamate from glutamate and hydroxylamine with crude tissue preparations is shown to give values far in excess of the true glutamine synthetase activity of the tissue. This is due to the generation of pyruvate, which reacts with hyd...
متن کاملRegulation of synthesis of glutamine synthetase by adenylylated glutamine synthetase.
We have examined three mutants of Klebsiella aerogenes whose genetic lesions (glnB, glnD, and glnE) are in loci unlinked to the structural gene for glutamine sythetase (glnA) and in which the control of both the level and state of adenylylation of glutamine synthetase is altered. Each mutation alters a different component of the adenylylation system of glutamine synthetase [L-glutamate:ammonia ...
متن کاملThe glutamine synthetase of Prevotella bryantii B(1)4 is a family III enzyme (GlnN) and glutamine supports growth of mutants lacking glutamate dehydrogenase activity.
Prevotella spp. are believed to play a central role in ruminal nitrogen metabolism, but little is understood about the genetics and biochemistry of nitrogen assimilation and regulation in these bacteria. The gene encoding a family III glutamine synthetase (GSIII, glnN) in Prevotella bryantii B(1)4 was cloned by Escherichia coli mutant complementation, and enzyme assays as well as Northern blot ...
متن کاملRegulation of glutamine synthetase activity of hepatoma tissue culture cells by glutamine and dexamethasone.
Regulation of glutamine synthetase activity was studied in a glutamine-independent subclone of the hepatoma tissue culture cell line. In cells of this subclone glutamine synthetase activity was elevated by the synthetic corticosteroid hormone, dexamethasone, and depressed by glutamine. The increase in glutamine synthetase activity due to the addition of dexamethasone and that due to removal of ...
متن کاملOxidative Modification of Glutamine Synthetase
The first step in the proteolytic degradation of bacterial glutamine synthetase is a mixed function oxidation of one of the 16 histidine residues in the glutamine synthetase subunit (Levine, R. L. (1983) J. Biol. Chem. 258, 11823-11827). A model system, consisting of oxygen, a metal ion, and ascorbic acid, mimics the bacterial system in mediating the oxidative modification of glutamine syntheta...
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ژورنال
عنوان ژورنال: Nature Cell Biology
سال: 2015
ISSN: 1465-7392,1476-4679
DOI: 10.1038/ncb3272